Analysis of fullerene-based nanomaterial in serum matrix by CE

Electrophoresis. 2007 May;28(10):1518-24. doi: 10.1002/elps.200600724.

Abstract

With the increasing interest in using nanoparticles as vehicles for drug delivery and image contrast agents, there is a need to develop assays for their detection and quantitation in complex matrices to facilitate monitoring their biodistribution. In this study, we developed a CE approach for the analysis of two nanoparticles: carboxyfullerene (C3) and dendrofullerene (DF1) in both standard solutions and a serum matrix. These highly soluble, charged C(60) derivatives were characterized by CZE using either a bare or dynamically coated fused-silica capillaries. The resolution of both nanoparticles was slightly lower with the coated capillary; however, their migration times were faster. While separation of the DF1 nanoparticles using MEKC resulted in a greater number of observable peaks, the peak profile of C3 was basically unchanged regardless of whether SDS micelles were added to the running buffers or not. The MEKC and/or CZE assays were then used to quantitate the C3 and DF1 nanoparticles in spiked human serum samples. The quantitation of the nanoparticles was linear from 0-500 microg/mL with detection limits ranging from 0.5 to 6 microg/mL.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Buffers
  • Calibration / standards
  • Chromatography, Micellar Electrokinetic Capillary / methods*
  • Electrophoresis, Capillary / instrumentation
  • Electrophoresis, Capillary / methods*
  • Fullerenes / analysis*
  • Fullerenes / blood*
  • Humans
  • Hydrogen-Ion Concentration
  • Indicators and Reagents
  • Nanostructures / analysis*
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Serum / chemistry

Substances

  • Buffers
  • Fullerenes
  • Indicators and Reagents