Photochemical DNA activation

Org Lett. 2007 May 10;9(10):1903-6. doi: 10.1021/ol070455u. Epub 2007 Apr 21.


A new photocaged nucleoside was synthesized and incorporated into DNA with the use of standard synthesis conditions. This approach enabled the disruption of specific H-bonds and allowed for the analysis of their contribution to the activity of a DNAzyme. Brief irradiation with nonphotodamaging UV light led to rapid decaging and almost quantitative restoration of DNAzyme activity. The developed strategy has the potential to find widespread application in the light-induced regulation of oligonucleotide function.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • DNA, Catalytic / chemistry*
  • DNA, Catalytic / metabolism*
  • Molecular Sequence Data
  • Molecular Structure
  • Photochemistry
  • RNA / metabolism
  • Substrate Specificity


  • DNA, Catalytic
  • RNA