Evaluation of the effect of cola drinks on bone mineral density and associated factors

Basic Clin Pharmacol Toxicol. 2007 May;100(5):334-8. doi: 10.1111/j.1742-7843.2007.00053.x.


The aim of the study was to determine bone mineral density changes caused by consumption of cola drinks and the associated factors. Thirty Sprague-Dawley rats were divided into four groups. Groups 1 and 2, consisting of 10 male and 10 female rats, respectively, were provided with as much food, water and cola drinks as they wanted. Groups 3 and 4, consisting of five rats each, received only rat chow and water. The bone mineral density of the rats was measured using dual energy X-ray absorptiometry at the end of 30 days. The blood values and weights of the animals were also determined. The oesophagus and kidneys were removed for histopathological examination. The weight gain was higher in the groups consuming cola drinks than the control group rats (P < 0.05). Water consumption decreased 5.9 times while total fluid consumption increased 1.6-1.9 times in the group consuming cola drinks. No significant change was detected in the blood calcium levels. There was a significant decrease in the bone mineral density of test groups when compared to the control groups (P < 0.05). While we did not detect any pathological oesophageal changes in the rats consuming cola drinks, examination of the kidneys revealed general glomerular congestion and intertubular bleeding. We suggest that the decrease in bone mineral density might be related to the renal damage caused by cola drinks in addition to other related factors.

MeSH terms

  • Absorptiometry, Photon
  • Animals
  • Blood Chemical Analysis
  • Body Weight / drug effects
  • Bone Density / drug effects*
  • Calcification, Physiologic / drug effects*
  • Carbonated Beverages / adverse effects*
  • Drinking / drug effects
  • Esophagus / drug effects
  • Esophagus / pathology
  • Female
  • Femur / diagnostic imaging
  • Femur / drug effects*
  • Femur / metabolism
  • Kidney / drug effects
  • Kidney / pathology
  • Male
  • Osteogenesis / drug effects*
  • Rats
  • Rats, Sprague-Dawley