Quantitative proteomic assessment of very early cellular signaling events

Nat Biotechnol. 2007 May;25(5):566-8. doi: 10.1038/nbt1301. Epub 2007 Apr 22.


Technical limitations have prevented proteomic analyses of events occurring less than 30 s after signal initiation. We developed an automated, continuous quench-flow system allowing quantitative proteomic assessment of very early cellular signaling events (qPACE) with a time resolution of 1 s. Using this technique, we determined that autophosphorylation of the epidermal growth factor receptor occurs within 1 s after ligand stimulation and is followed rapidly by phosphorylation of the downstream signaling intermediates Src homologous and collagen-like protein and phospholipase C gamma 1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ErbB Receptors / metabolism*
  • Fluorescence Recovery After Photobleaching / methods*
  • Metabolic Clearance Rate
  • Microscopy, Fluorescence / methods*
  • Phosphorylation
  • Proteome / metabolism*
  • Signal Transduction / physiology*
  • Time Factors


  • Proteome
  • ErbB Receptors