Abstract
Methods for introducing bioorthogonal functionalities into proteins have become central to protein engineering efforts. Here we describe a method for the site-specific introduction of aldehyde groups into recombinant proteins using the 6-amino-acid consensus sequence recognized by the formylglycine-generating enzyme. This genetically encoded 'aldehyde tag' is no larger than a His(6) tag and can be exploited for numerous protein labeling applications.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Alanine / analogs & derivatives
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Alanine / genetics
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Alanine / metabolism
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Aldehydes / metabolism*
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Amino Acid Sequence
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Animals
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Consensus Sequence
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Conserved Sequence
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Glycine / analogs & derivatives
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Glycine / genetics
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Glycine / metabolism
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Humans
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Molecular Sequence Data
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Peptide Fragments / chemistry
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Protein Processing, Post-Translational
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Proteins / chemistry
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Proteins / genetics
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Proteins / metabolism*
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Sequence Tagged Sites
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Sulfatases / chemistry
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Sulfatases / genetics
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Sulfatases / metabolism
Substances
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Aldehydes
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Peptide Fragments
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Proteins
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C(alpha)-formylglycine
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Sulfatases
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Alanine
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Glycine
Associated data
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PubChem-Substance/24420952
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PubChem-Substance/24420953