Introducing genetically encoded aldehydes into proteins

Nat Chem Biol. 2007 Jun;3(6):321-2. doi: 10.1038/nchembio878. Epub 2007 Apr 22.

Abstract

Methods for introducing bioorthogonal functionalities into proteins have become central to protein engineering efforts. Here we describe a method for the site-specific introduction of aldehyde groups into recombinant proteins using the 6-amino-acid consensus sequence recognized by the formylglycine-generating enzyme. This genetically encoded 'aldehyde tag' is no larger than a His(6) tag and can be exploited for numerous protein labeling applications.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Alanine / analogs & derivatives
  • Alanine / genetics
  • Alanine / metabolism
  • Aldehydes / metabolism*
  • Amino Acid Sequence
  • Animals
  • Consensus Sequence
  • Conserved Sequence
  • Glycine / analogs & derivatives
  • Glycine / genetics
  • Glycine / metabolism
  • Humans
  • Molecular Sequence Data
  • Peptide Fragments / chemistry
  • Protein Processing, Post-Translational
  • Proteins / chemistry
  • Proteins / genetics
  • Proteins / metabolism*
  • Sequence Tagged Sites
  • Sulfatases / chemistry
  • Sulfatases / genetics
  • Sulfatases / metabolism

Substances

  • Aldehydes
  • Peptide Fragments
  • Proteins
  • C(alpha)-formylglycine
  • Sulfatases
  • Alanine
  • Glycine

Associated data

  • PubChem-Substance/24420952
  • PubChem-Substance/24420953