A yeast two-hybrid smart-pool-array system for protein-interaction mapping

Nat Methods. 2007 May;4(5):405-7. doi: 10.1038/nmeth1042. Epub 2007 Apr 22.

Abstract

We present here a new two-hybrid smart pool array (SPA) system in which, instead of individual activation domain strains, well-designed activation domain pools are screened in an array format that allows built-in replication and prey-bait deconvolution. Using this method, a Saccharomyces cerevisiae genome SPA increases yeast two-hybrid screening efficiency by an order of magnitude.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Genome, Fungal / genetics
  • Protein Interaction Mapping / methods*
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae Proteins / genetics*
  • Two-Hybrid System Techniques*

Substances

  • Saccharomyces cerevisiae Proteins