High Level of Genetic Heterogeneity in S and P Genes of Genotype D Hepatitis B Virus

Virology. 2007 Aug 15;365(1):113-24. doi: 10.1016/j.virol.2007.03.015. Epub 2007 Apr 23.

Abstract

The genetic heterogeneity of hepatitis B virus (HBV) genotypes and subgenotypes was investigated by directly sequencing amplified PreS, S and P genes of HBV isolates obtained from the plasma of 99 subjects with chronic HBV infection. Genotype D showed the greatest intragenotypic and intrasubgenotypic divergence: in particular, the a determinant was mutated in 58.2% of the genotype D patients, two of whom showed prototypic vaccine-induced escape mutants at codon 145. Moreover, five sites under significant positive selection were found in the S protein of the D isolates: one in the a determinant and four in the highly hydrophobic C terminal. Our results suggest that careful surveillance of vaccine-induced escape mutants should be considered in populations with highly frequent genotype D infections, and raise questions concerning the possible relationship between the genetic heterogeneity, host immunity and pathogenicity of this HBV genotype.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • DNA, Viral / analysis
  • Female
  • Genetic Heterogeneity*
  • Genotype
  • Hepatitis B Surface Antigens / analysis*
  • Hepatitis B Surface Antigens / chemistry
  • Hepatitis B Surface Antigens / genetics*
  • Hepatitis B Virus, Duck / classification
  • Hepatitis B Virus, Duck / genetics*
  • Humans
  • Male
  • Molecular Sequence Data
  • Phylogeny
  • Sequence Analysis, DNA

Substances

  • DNA, Viral
  • Hepatitis B Surface Antigens

Associated data

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