Cerebrospinal fluid from amyotrophic lateral sclerosis patients causes fragmentation of the Golgi apparatus in the neonatal rat spinal cord

Amyotroph Lateral Scler. 2007 Apr;8(2):79-82. doi: 10.1080/08037060601145489.

Abstract

We have previously shown in our laboratory that cerebrospinal fluid from ALS patients (ALS-CSF) contains putative toxic factor(s). In the present study we determined the effect of ALS-CSF on the integrity of the Golgi apparatus of spinal motor neurons in the neonatal rats. CSF was injected intrathecally into three-day-old rat pups and subsequently the ultrastructural changes in the motor neurons were studied after 48 h, 1, 2 and 3 weeks. We observed that ALS-CSF causes fragmentation of the Golgi apparatus in a considerable number of motor neurons in the spinal cord. This was further confirmed when motor neurons were stained with an antibody against a medial Golgi protein (MG160). Thus, we suggest that the putative toxin(s) present in ALS-CSF may cause impairment in the protein processing leading to motor neuron death.

MeSH terms

  • Amyotrophic Lateral Sclerosis / cerebrospinal fluid*
  • Animals
  • Animals, Newborn
  • Cerebrospinal Fluid Proteins / administration & dosage*
  • Golgi Apparatus / drug effects*
  • Golgi Apparatus / pathology
  • Humans
  • Injections, Spinal
  • Motor Neurons / drug effects*
  • Motor Neurons / pathology*
  • Rats
  • Rats, Wistar
  • Spinal Cord / drug effects*
  • Spinal Cord / pathology*

Substances

  • Cerebrospinal Fluid Proteins