[6]-Gingerol prevents UVB-induced ROS production and COX-2 expression in vitro and in vivo

Free Radic Res. 2007 May;41(5):603-14. doi: 10.1080/10715760701209896.

Abstract

[6]-Gingerol, a naturally occurring plant phenol, is one of the major components of fresh ginger (Zingiber officinale Roscoe, Zingiberaceae) and has diverse pharmacologic effects. Here, we describe its novel anti-oxidant, anti-apoptotic, and anti-inflammatory activities in vitro and in vivo. In vitro, pre-treatment with [6]-gingerol reduced UVB-induced intracellular reactive oxygen species levels, activation of caspase-3, -8, -9, and Fas expression. It also reduced UVB-induced expression and transactivation of COX-2. Translocation of NF-kappaB from cytosol to nucleus in HaCaT cells was inhibited by [6]-gingerol via suppression of IkappaBalpha phosphorylation (ser-32). Examination by EMSAs and immunohistochemistry showed that topical application of [6]-gingerol (30 microM) prior to UVB irradiation (5 kJ/m(2)) of hairless mice, also inhibited the induction of COX-2 mRNA and protein, as well as NF-kappaB translocation. These results suggest that [6]-gingerol could be an effective therapeutic agent providing protection against UVB-induced skin disorders.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Northern
  • Blotting, Western
  • Catechols
  • Cell Line
  • Cell Nucleus / drug effects
  • Cell Nucleus / metabolism
  • Cell Nucleus / radiation effects
  • Chemoprevention
  • Cyclooxygenase 2 / genetics
  • Cyclooxygenase 2 / metabolism*
  • Cyclooxygenase 2 / radiation effects
  • Cytosol / drug effects
  • Cytosol / metabolism
  • Cytosol / radiation effects
  • Electrophoretic Mobility Shift Assay
  • Fatty Alcohols / pharmacology*
  • Humans
  • Keratinocytes / drug effects
  • Keratinocytes / physiology
  • Keratinocytes / radiation effects*
  • Male
  • Mice
  • Mice, Hairless
  • Mutagens / pharmacology*
  • NF-kappa B / metabolism
  • Phosphorylation / drug effects
  • Phosphorylation / radiation effects
  • Protein Transport
  • Reactive Oxygen Species / metabolism*
  • Reactive Oxygen Species / radiation effects
  • Skin / drug effects
  • Skin / radiation effects
  • Skin Neoplasms / prevention & control*
  • Ultraviolet Rays*
  • Zingiber officinale / chemistry

Substances

  • Catechols
  • Fatty Alcohols
  • Mutagens
  • NF-kappa B
  • Reactive Oxygen Species
  • gingerol
  • Cyclooxygenase 2