Cytoplasmic/nuclear localization of tuberin in different cell lines

Amino Acids. 2007 Nov;33(4):575-9. doi: 10.1007/s00726-007-0541-0. Epub 2007 Apr 26.


Tuberous sclerosis (TSC) is an autosomal dominantly inherited disease affecting 1 in 6000 individuals. The TSC gene products, hamartin and tuberin, form a complex, of which tuberin is assumed to be the functional component being involved in a wide variety of different cellular processes. Tuberin has been demonstrated to be localized to both, the cytoplasm and the nucleus. The cytoplasmic/nuclear localization of tuberin is known to be regulated by the serine/threonine protein kinase Akt. Akt also regulates the cytoplasmic/nuclear localization of the cyclin-dependent kinase inhibitor p27. In this study the localization of these two Akt-regulated proteins was analysed in different cell lines.

MeSH terms

  • Animals
  • Cell Line
  • Cell Nucleus / metabolism*
  • Cyclin-Dependent Kinase Inhibitor p27 / isolation & purification
  • Cyclin-Dependent Kinase Inhibitor p27 / metabolism
  • Cytoplasm / metabolism*
  • HeLa Cells
  • Humans
  • Mice
  • NIH 3T3 Cells
  • Phosphorylation
  • Proto-Oncogene Proteins c-akt / metabolism
  • Tuberous Sclerosis / metabolism*
  • Tuberous Sclerosis Complex 2 Protein
  • Tumor Suppressor Proteins / isolation & purification
  • Tumor Suppressor Proteins / metabolism*


  • TSC2 protein, human
  • Tuberous Sclerosis Complex 2 Protein
  • Tumor Suppressor Proteins
  • Cyclin-Dependent Kinase Inhibitor p27
  • Proto-Oncogene Proteins c-akt