Overexpression of protein kinase C alpha mRNA in human hepatocellular carcinoma: a potential marker of disease prognosis

Clin Chim Acta. 2007 Jul;382(1-2):54-8. doi: 10.1016/j.cca.2007.03.018. Epub 2007 Mar 28.


Background: Members of the protein kinase C (PKC) isoenzyme family play a central role in the tumorigenesis of several tissues. However, little is known about subtype specific intracellular expression of PKC in human hepatocellular carcinomas.

Methods: We investigated PKC isoforms mRNA expression in 42 HCC specimens using reverse transcription polymerase chain reaction analysis, and the correlation between PKC isoforms expression and clinicopathologic parameters.

Results: We found that PKCalpha, PKCdelta and PKCiota mRNA were significantly increased in HCCs as compared to the corresponding non-cancerous liver tissues. PKCalpha expression also significantly correlated with tumor size (P<0.05) and TNM stage (P<0.05), but PKCdelta and PKCiota did not. The log-rank analysis revealed that patients with higher PKCalpha mRNA expression in the HCC tissues had significantly shorter survival rate than patients with lower PKCalpha mRNA expression (P<0.01).

Conclusions: Our results suggested that the PKCalpha may be a prognostic factor for the survival of patients with HCC.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Biomarkers
  • Carcinoma, Hepatocellular / diagnosis
  • Carcinoma, Hepatocellular / enzymology*
  • Female
  • Humans
  • Isoenzymes / genetics
  • Isoenzymes / metabolism
  • Liver Neoplasms / diagnosis
  • Liver Neoplasms / enzymology*
  • Male
  • Middle Aged
  • Prognosis
  • Protein Kinase C / genetics
  • Protein Kinase C / metabolism
  • Protein Kinase C-alpha / genetics*
  • Protein Kinase C-alpha / metabolism
  • Protein Kinase C-delta / genetics
  • Protein Kinase C-delta / metabolism
  • RNA, Messenger / metabolism*


  • Biomarkers
  • Isoenzymes
  • RNA, Messenger
  • Protein Kinase C
  • Protein Kinase C-alpha
  • Protein Kinase C-delta
  • protein kinase C lambda