[Preparation and identification of a single-chain antibody fragment against high pathogenic H5N1 avian influenza virus]

Sheng Wu Gong Cheng Xue Bao. 2007 Mar;23(2):292-6. doi: 10.1016/s1872-2075(07)60026-0.
[Article in Chinese]


Previously, an mAb 10F7 was developed against H5N1 hemagglutinin, which was highly specific to 34 different H5N1 strains and showed good neutralizing activity. In the present study, the single-chain fragment of the antibody was cloned into a prokaryotic vector and then expressed in E. coli. The activity of the scFv was tested in hemagglution inhibition and neutralization experiment. Two H5N1 virus strains were inhibited to bind erythrocyte cells by the scFv while the H9 virus was not. Also, five H5N1 virus strains were neutralized during infecting MDCK cells. These results showed an approachable method for developing therapeutic antibody to H5N1 virus.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies, Viral / genetics
  • Antibodies, Viral / immunology*
  • Antibodies, Viral / metabolism
  • Antibody Specificity / immunology
  • Birds / virology
  • Cell Line
  • Cell Line, Tumor
  • Electrophoresis, Polyacrylamide Gel
  • Influenza A Virus, H5N1 Subtype / immunology*
  • Influenza in Birds / virology
  • Neutralization Tests
  • Recombinant Proteins / immunology
  • Recombinant Proteins / metabolism
  • Single-Chain Antibodies / genetics
  • Single-Chain Antibodies / immunology*
  • Single-Chain Antibodies / metabolism


  • Antibodies, Viral
  • Recombinant Proteins
  • Single-Chain Antibodies