Chemiluminescence intensities and spectra of luminol oxidation by sodium hypochlorite in the presence of hydrogen peroxide

J Biolumin Chemilumin. 1991 Jul-Sep;6(3):189-92. doi: 10.1002/bio.1170060309.


Hydrogen peroxide amplifies the chemiluminescence in the oxidation of luminol by sodium hypochlorite. A linear relationship between concentration of hydrogen peroxide and light intensity was found in the concentration range 5 x 10(-8)-7.5 x 10(-6) mol/l. At 7.5 x 10(-6) mol/l H2O2 the chemiluminescence is amplified 550-fold. The chemiluminescence spectra of these reactions have a wavelength maximum at 431 nm independent of the concentration of hydrogen peroxide. The results indicate that hydrogen peroxide is a necessary component in the chemiluminescent oxidation of the luminol by sodium hypochlorite.

MeSH terms

  • Hydrogen Peroxide / chemistry
  • Luminescent Measurements*
  • Luminol / chemistry*
  • Models, Biological
  • Oxidation-Reduction
  • Phagocytosis / physiology
  • Sodium Hypochlorite / chemistry
  • Spectrophotometry


  • Luminol
  • Hydrogen Peroxide
  • Sodium Hypochlorite