Leptin affects proliferation-, apoptosis- and protein kinase A-related peptides in human ovarian granulosa cells

Physiol Res. 2008;57(3):437-442. doi: 10.33549/physiolres.931153. Epub 2007 Apr 25.


The aim of our in vitro studies was to understand the role of leptin in controlling proliferation, apoptosis, and protein kinase A (PKA) in human ovarian cells. We analyzed the in vitro effects of leptin (0, 1, 10 or 100 ng/ml) on the accumulation of proliferation-related peptides (PCNA, cyclin B1), apoptosis-associated peptide (Bax) and the intracellular signaling molecule PKA in cultured human granulosa cells using immunocytochemistry and Western immunoblotting. It was observed that leptin stimulated in a dose-dependent manner the accumulation of PCNA (at doses 1-100 ng/ml), cyclin B1 (at doses 10 or 100 ng/ml), Bax (at doses 10 or 100 ng/ml) and PKA (at doses 1-100 ng/ml) in cultured human ovarian cells. These observations suggest the ability of leptin to control directly human ovarian cell functions: proliferation, apoptosis, and intracellular messenger PKA.

MeSH terms

  • Adult
  • Apoptosis*
  • Blotting, Western
  • Cell Cycle
  • Cell Proliferation*
  • Cells, Cultured
  • Cyclic AMP-Dependent Protein Kinases / metabolism*
  • Cyclin B / metabolism
  • Cyclin B1
  • Female
  • Granulosa Cells / enzymology*
  • Granulosa Cells / immunology
  • Granulosa Cells / pathology
  • Humans
  • Immunohistochemistry
  • Leptin / metabolism*
  • Proliferating Cell Nuclear Antigen / metabolism
  • Recombinant Proteins / metabolism
  • Signal Transduction
  • bcl-2-Associated X Protein / metabolism


  • BAX protein, human
  • CCNB1 protein, human
  • Cyclin B
  • Cyclin B1
  • Leptin
  • Proliferating Cell Nuclear Antigen
  • Recombinant Proteins
  • bcl-2-Associated X Protein
  • Cyclic AMP-Dependent Protein Kinases