A set of BEST triple-resonance experiments for time-optimized protein resonance assignment

J Magn Reson. 2007 Jul;187(1):163-9. doi: 10.1016/j.jmr.2007.04.002. Epub 2007 Apr 13.


A series of sequential, intra-residue, and bi-directional BEST H-N-CA, H-N-CO, and H-N-CB pulse sequences is presented that extends the BEST concept introduced recently for fast multidimensional protein NMR [Schanda et al., J. Am. Chem. Soc. 128 (2006) 9042] to the complete set of experiments required for sequential resonance assignment. We demonstrate for the protein ubiquitin that 3D BEST H-N-C correlation spectra can be recorded on a 600MHz NMR spectrometer equipped with a cryogenic probe in only a few minutes of acquisition time with sufficient sensitivity to detect all expected cross peaks.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Algorithms
  • Magnetic Resonance Spectroscopy / statistics & numerical data*
  • Models, Statistical
  • Proteins / chemistry*
  • Ubiquitin / chemistry


  • Proteins
  • Ubiquitin