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, 73 (13), 4379-84

Repeat Domain Diversity of avrBs3-like Genes in Ralstonia Solanacearum Strains and Association With Host Preferences in the Field

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Repeat Domain Diversity of avrBs3-like Genes in Ralstonia Solanacearum Strains and Association With Host Preferences in the Field

Holger Heuer et al. Appl Environ Microbiol.

Abstract

Genes homologous to avrBs3 of Xanthomonas were detected in 309 strains of Ralstonia solanacearum biovars 3, 4, and 5 but not biovar 1 or 2. A statistically significant association between the originating plant species and internal repeats of the gene was found. Sequences of repeats and variation between nearly clonal strains revealed evidence of frequent recombination.

Figures

FIG. 1.
FIG. 1.
Agarose gel electrophoresis of PCR products (A) and AluI restriction patterns (B) from brg11- and hpx17-like genes of R. solanacearum strains. Lanes: M, size marker; 0, no product; a to e, representative strains of the five size classes.
FIG. 2.
FIG. 2.
Amino acid neighbor-joining tree of single internal repeats of brg11, hpx17, hax2, and homologues of R. solanacearum strains SD58 (type a), GX526 (type b2), GD52 (type c), FJ49 (type d1), ZJ1993Bn1 (type d2), GX57 and ICPM11121 (type d3), FJP16 (type d4), and UW360 (type e). In total, 123 repeats were analyzed. Similar repeats were grouped. Repeats were numbered according to the position in brg11 +1 to + 9 (from the 5′ end) and −1 to −9 (from the 3′ end). Bootstrap consensus tree, n = 100, PAM.

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