Effect of frequency on insulin response to electric field stress

J Phys Chem B. 2007 May 24;111(20):5748-56. doi: 10.1021/jp067248g. Epub 2007 May 2.

Abstract

There are many unanswered questions regarding the precise way in which proteins respond to external stress. Since the function of proteins is critically linked to their three-dimensional structures, exposure to any form of stress which may induce changes in conformation can potentially initiate severe cellular dysfunction. This is particularly relevant with regard to the increasing presence of electromagnetic devices in today's environment and the possible effects on human health. Previously, we investigated the effect of electric field of various strengths on insulin chain-B under static and oscillating conditions. This paper expands on our previous work by subjecting the peptide to an oscillating electric field of different frequencies. We observed a frequency-dependent effect where the application of lower-frequency oscillating fields resulted in static-field-like behavior of the peptide, whereby the intrinsic flexibility of the protein is constrained, thus potentially restricting access to the protein's active state.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Electrochemistry / methods*
  • Electromagnetic Fields
  • Insulin / chemistry*
  • Models, Molecular
  • Protein Conformation

Substances

  • Insulin