Radioprotective and antioxidant activity of fractionated extracts of berries of Hippophae rhamnoides

J Med Food. 2007 Mar;10(1):101-9. doi: 10.1089/jmf.2006.007.

Abstract

Plants are an abundant source of medicinal compounds, some of which are useful in combating free radical-mediated oxidative stress. In the present study, initially two fractions designated REC-1001 (flavonoid-rich fraction) and REC-1002 (flavonoid-poor fraction) of Hippophae rhamnoides were screened on the basis of their reducing power in the aqueous phase. REC-1001 was selected for further study, since it exhibited 27.38 times higher antioxidant activity than REC-1002. REC-1001 also showed significant (P < .05) membrane protection potential at 50 microg/mL, which was attributed to its ability to scavenge peroxyl radicals (64.82 +/- 1.25% scavenging within 1,440 min). A significant (P < .05) difference of 67.02% in free radical scavenging activity at 1,000 ng/mL between REC-1001 and vitamin E demonstrated the extract fraction's worth in radiation protection. Such activities were attributed to the presence of quercetin, isorhamnetin, and kaempferol in this fraction. Further, REC-1001 was found to be nontoxic up to 200 mg/kg of body weight. This research suggests that the REC-1001 fraction of H. rhamnoides extract is a safe and effective antioxidant nutraceutical product.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / pharmacology*
  • Chemical Fractionation
  • Flavonols / analysis
  • Free Radical Scavengers / pharmacology
  • Fruit / chemistry*
  • Hippophae / chemistry*
  • Kaempferols / analysis
  • Lethal Dose 50
  • Mice
  • Peroxides
  • Plant Extracts / chemistry*
  • Plant Extracts / pharmacology*
  • Plant Extracts / toxicity
  • Quercetin / analysis
  • Radiation-Protective Agents / pharmacology*

Substances

  • Antioxidants
  • Flavonols
  • Free Radical Scavengers
  • Kaempferols
  • Peroxides
  • Plant Extracts
  • Radiation-Protective Agents
  • 3-methylquercetin
  • perhydroxyl radical
  • kaempferol
  • Quercetin