Conformational changes of glucose/galactose-binding protein illuminated by open, unliganded, and ultra-high-resolution ligand-bound structures

Protein Sci. 2007 Jun;16(6):1032-41. doi: 10.1110/ps.062707807. Epub 2007 May 1.

Abstract

D-Glucose/D-Galactose-binding protein (GGBP) mediates chemotaxis toward and active transport of glucose and galactose in a number of bacterial species. GGBP, like other periplasmic binding proteins, can exist in open (ligand-free) and closed (ligand-bound) states. We report a 0.92 angstroms resolution structure of GGBP from Escherichia coli in the glucose-bound state and the first structure of an open, unbound form of GGBP (at 1.55 angstroms resolution). These structures vary in the angle between the two structural domains; the observed difference of 31 degrees arises from torsion angle changes in a three-segment hinge. A comparison with the closely related periplasmic receptors, ribose- and allose-binding proteins, shows that the GGBP hinge residue positions that undergo the largest conformational changes are different. Furthermore, the high-quality data collected for the atomic resolution glucose-bound structure allow for the refinement of specific hydrogen atom positions, the assignment of alternate side chain conformations, the first description of CO(2) trapped after radiation-induced decarboxylation, and insight into the role of the exo-anomeric effect in sugar binding. Together, these structures provide insight into how the hinge-bending movement of GGBP facilitates ligand binding, transport, and signaling.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding Sites
  • Crystallography, X-Ray
  • Escherichia coli Proteins / chemistry*
  • Escherichia coli Proteins / metabolism
  • Galactose / chemistry*
  • Galactose / metabolism
  • Glucose / chemistry*
  • Glucose / metabolism
  • Magnetic Resonance Spectroscopy
  • Models, Molecular
  • Molecular Structure
  • Monosaccharide Transport Proteins / chemistry*
  • Monosaccharide Transport Proteins / metabolism
  • Protein Binding
  • Protein Structure, Secondary
  • Protein Structure, Tertiary

Substances

  • Escherichia coli Proteins
  • Monosaccharide Transport Proteins
  • mglB protein, E coli
  • Glucose
  • Galactose

Associated data

  • PDB/2FVY
  • PDB/2FW0