Virgibacillus olivae sp. nov., isolated from waste wash-water from processing of Spanish-style green olives

Int J Syst Evol Microbiol. 2007 May;57(Pt 5):906-910. doi: 10.1099/ijs.0.64550-0.


Four bacterial strains (E(30)8(T), E(55)49, I(30)77 and N(30)129) were isolated from the residual wash-water produced during the processing of Spanish-style green table olives. The isolates were subjected to a polyphasic taxonomic study using phenotypic, phylogenetic and genotypic methods. The bacteria were Gram-positive, spore-forming rods. Moreover, they were heterotrophs that were able to utilize cellobiose, glucose, mannose and rhamnose as carbon sources. The G+C content of their genomic DNA ranged from 30.7 to 33.4 mol%. The major cellular fatty acids found in strain E(30)8(T) were iso-C(15 : 0), anteiso-C(15 : 0), iso-C(17 : 0) and anteiso-C(17 : 0). DNA-DNA hybridization shows 76.2-88.3 % relatedness among the four strains. The 16S rRNA gene sequence of isolate E(30)8(T) shows that it belongs to the genus Virgibacillus, with the highest sequence similarity (99 %) to Virgibacillus marismortui 123(T). However, phenotypic differences and DNA-DNA relatedness between strain E(30)8(T) and V. marismortui ATCC 700626(T) of less than 47 % suggest the placement of these strains within a novel species of the genus Virgibacillus. The name Virgibacillus olivae sp. nov. is proposed, with strain E(30)8(T) (=LMG 23503(T)=DSM 18098(T)) as the type strain.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacillaceae / chemistry
  • Bacillaceae / classification*
  • Bacillaceae / genetics
  • Bacillaceae / isolation & purification*
  • Base Composition
  • Carbohydrate Metabolism
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Fatty Acids / analysis
  • Food Microbiology*
  • Genes, rRNA / genetics
  • Gentian Violet
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Olea / microbiology
  • Phenazines
  • Phylogeny
  • RNA, Bacterial / genetics
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Sequence Homology, Nucleic Acid
  • Water Microbiology


  • DNA, Bacterial
  • DNA, Ribosomal
  • Fatty Acids
  • Gram's stain
  • Phenazines
  • RNA, Bacterial
  • RNA, Ribosomal, 16S
  • Gentian Violet

Associated data

  • GENBANK/DQ139839