Halalkalibacillus halophilus gen. nov., sp. nov., a novel moderately halophilic and alkaliphilic bacterium isolated from a non-saline soil sample in Japan

Int J Syst Evol Microbiol. 2007 May;57(Pt 5):1081-1085. doi: 10.1099/ijs.0.64830-0.

Abstract

A moderately halophilic and alkaliphilic bacterium, designated strain BH2(T), was isolated from non-saline garden soil in Saitama, Japan. Cells of strain BH2(T) were motile, aerobic, rod-shaped and Gram-positive and contained A1gamma, meso-diaminopimelic acid-type murein. Growth occurred in 5.0-25 % (w/v) NaCl (optimum, 10-15 %, w/v), at pH 5.5-10.0 (optimum, pH 8.5-9.0) and at 20-40 degrees C. The predominant isoprenoid quinone was menaquinone-7. The major cellular fatty acids were ai-C(15 : 0), i-C(16 : 0), ai-C(17 : 0) and i-C(15 : 0). The G+C content of the total DNA of strain BH2(T) was 35.1+/-0.4 mol% (+/-sd; n=5). The phylogenetic distance from species with validly published names was less than 94.1 %. The phylogenetic and phenotypic characteristics indicated that strain BH2(T) represents a novel genus and species, for which the name Halalkalibacillus halophilus gen. nov., sp. nov. is proposed. The type strain is BH2(T) (=JCM 14192(T)=DSM 18494(T)).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacillaceae / chemistry
  • Bacillaceae / classification*
  • Bacillaceae / isolation & purification*
  • Bacillaceae / physiology
  • Bacterial Typing Techniques
  • Base Composition
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Fatty Acids / analysis
  • Genes, rRNA
  • Gentian Violet / metabolism
  • Hydrogen-Ion Concentration
  • Japan
  • Molecular Sequence Data
  • Movement
  • Phenazines / metabolism
  • Phylogeny
  • Quinones / analysis
  • RNA, Bacterial / genetics
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Sequence Homology, Nucleic Acid
  • Sodium Chloride / metabolism
  • Soil Microbiology*
  • Temperature

Substances

  • DNA, Bacterial
  • DNA, Ribosomal
  • Fatty Acids
  • Gram's stain
  • Phenazines
  • Quinones
  • RNA, Bacterial
  • RNA, Ribosomal, 16S
  • Sodium Chloride
  • Gentian Violet

Associated data

  • GENBANK/AB264529