The anti-thrombotic active constituents from Centella asiatica

Biol Pharm Bull. 2007 May;30(5):935-40. doi: 10.1248/bpb.30.935.


The in vitro effects of a methanol extract from the aerial parts of Centella asiatica on shear-induced platelet activation and coagulation were assessed after oral administration to rats, by subjecting non-anticoagulated blood to haemostatometry. 3,5-Di-O-caffeoyl quinic acid, 1,5-di-O-caffeoyl quinic acid, 3,4-di-O-caffeoyl quinic acid, 4,5-di-O-caffeoyl quinic acid, and chlorogenic acid, together with asiaticoside, kaempferol, quercetine, kaempferol-3-O-beta-D-glucoside and quercetin-3-O-beta-D-glucoside were all isolated from the methanol extract. Amongst these, only 3,5-di-O-caffeoylquinic acid showed significant inhibition of shear-induced platelet activation and dynamic coagulation. The reactive curve of the inhibitory effect on the platelet reaction and the dynamic coagulation showed a bell-shape.

MeSH terms

  • Administration, Oral
  • Animals
  • Blood Coagulation / drug effects*
  • Blood Coagulation Tests / instrumentation
  • Blood Platelets / drug effects*
  • Blood Platelets / physiology
  • Centella / chemistry*
  • Fibrinolytic Agents / chemistry
  • Fibrinolytic Agents / isolation & purification
  • Fibrinolytic Agents / pharmacology*
  • Male
  • Methanol
  • Molecular Structure
  • Plant Components, Aerial / chemistry
  • Plant Extracts / chemistry
  • Plant Extracts / isolation & purification
  • Plant Extracts / pharmacology
  • Platelet Activation / drug effects*
  • Rats
  • Rats, Wistar
  • Structure-Activity Relationship


  • Fibrinolytic Agents
  • Plant Extracts
  • Methanol