Tag-mass: specific molecular imaging of transcriptome and proteome by mass spectrometry based on photocleavable tag

J Proteome Res. 2007 Jun;6(6):2057-67. doi: 10.1021/pr0700044. Epub 2007 May 4.


MALDI tissue imaging of tissues has become a promising technique for tracking biomarkers while determining their location and structural characterization. We have now developed specific targeting probes (oligonucleotides, antibodies), named Tag-Mass. This approach is based on probes modified with a photocleavable linker coupled with a tag cleaved and detected using mass spectrometry. Tag-Mass development is the key for a rapid, sensitive, and accurate approach to correlate levels of expression of different mRNA or proteins in diseases.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies / chemistry
  • Brain Chemistry
  • Mice
  • Molecular Probes / chemistry
  • Oligonucleotide Probes / chemistry
  • Photolysis
  • Proteins / analysis*
  • Proteome / genetics*
  • Proteomics / methods*
  • RNA, Messenger / analysis*
  • Rats
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
  • Transcription, Genetic


  • Antibodies
  • Molecular Probes
  • Oligonucleotide Probes
  • Proteins
  • Proteome
  • RNA, Messenger