Melatonin modifies calmodulin cell levels in MDCK and N1E-115 cell lines and inhibits phosphodiesterase activity in vitro

Brain Res. 1991 Aug 23;557(1-2):289-92. doi: 10.1016/0006-8993(91)90146-m.


The interaction between melatonin and calmodulin was explored. Calmodulin cell levels in MDCK and N1E-115 cells cultured with 10(-9) M melatonin were increased after 3 days but decreased after 6 days. Melatonin inhibited calmodulin-dependent phosphodiesterase and when either melatonin or [3H]melatonin was preincubated with calmodulin and separated by electrophoresis, comigration of calmodulin with the radioactivity as well as modification of the Ca2+ calmodulin shift were observed. The results point out that one of the mechanisms of action of melatonin is a calmodulin-melatonin interaction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calmodulin / biosynthesis
  • Calmodulin / metabolism*
  • Cell Line
  • Dogs
  • Electrophoresis
  • Melatonin / pharmacology*
  • Phosphodiesterase Inhibitors / pharmacology*


  • Calmodulin
  • Phosphodiesterase Inhibitors
  • Melatonin