Real-time microscopic visualization of tissue response to laser thermal therapy

J Biomed Opt. 2007 Mar-Apr;12(2):020501. doi: 10.1117/1.2714027.


We present methods for visualizing the dynamic response of biological samples to laser-induced heating. Our approach utilizes optical frequency-domain imaging to detect, spatially localize, and monitor unique dynamic signatures that arise within zones of active tissue denaturation. Since this information is precisely registered with high-resolution ( approximately 10 microm) cross sectional images, regions of thermally destroyed tissue can be mapped in relation to pre-existing morphology. Using porcine esophageal specimens ex vivo, we demonstrate that the extent and evolution of laser thermal damage can be assessed in real time.

Publication types

  • Evaluation Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Computer Systems
  • Esophagus / cytology*
  • Esophagus / surgery*
  • Hyperthermia, Induced / methods
  • Image Interpretation, Computer-Assisted / methods*
  • In Vitro Techniques
  • Laser Coagulation / methods*
  • Microscopy / methods*
  • Surgery, Computer-Assisted / methods*
  • Swine
  • Tomography, Optical Coherence / methods*