Mouse homologues of Shisa antagonistic to Wnt and Fgf signalings

Dev Biol. 2007 Jun 15;306(2):480-92. doi: 10.1016/j.ydbio.2007.03.028. Epub 2007 Mar 27.


In an effort to identify Otx2 targets in mouse anterior neuroectoderm we identified a gene, mShisa, which is homologous to xShisa1 that we previously reported as a head inducer in Xenopus. mShisa encodes an antagonist against both Wnt and Fgf signalings; it inhibits these signalings cell-autonomously as xShisa1 does. The mShisa expression is lost or greatly reduced in Otx2 mutant visceral endoderm, anterior mesendoderm and anterior neuroectoderm. However, mShisa mutants exhibited no defects in head development. Shisa is composed of five subfamilies, but normal head development in mShisa mutants is unlikely to be explained in terms of the compensation of mShisa deficiency by its paralogues or by known Wnt antagonists in anterior visceral endoderm and/or anterior mesendoderm.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Endoderm / metabolism
  • Fibroblast Growth Factors / antagonists & inhibitors
  • Fibroblast Growth Factors / genetics*
  • Gene Expression Regulation*
  • Gene Expression Regulation, Developmental*
  • Humans
  • Membrane Proteins / genetics*
  • Membrane Proteins / physiology*
  • Mesoderm / metabolism
  • Mice
  • Mice, Transgenic
  • Molecular Sequence Data
  • Wnt Proteins / antagonists & inhibitors
  • Wnt Proteins / genetics*
  • Xenopus


  • Membrane Proteins
  • Shisa protein, mouse
  • Wnt Proteins
  • Fibroblast Growth Factors