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. 2007 Jun 22;1154(1-2):469-72.
doi: 10.1016/j.chroma.2007.04.034. Epub 2007 Apr 20.

Improved Hydrophilic Interaction Chromatography Method for the Identification and Quantification of Glucosinolates

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Improved Hydrophilic Interaction Chromatography Method for the Identification and Quantification of Glucosinolates

Kristina L Wade et al. J Chromatogr A. .
Free PMC article

Abstract

An improved hydrophilic interaction liquid chromatography (HILIC) method has been developed to separate members of a closely related family of chemoprotective phytochemicals called glucosinolates. This method exploits the emergence of a second generation of HILIC chemistry, using a silica-based permanently zwitterionic stationary phase. These columns are more robust, durable, and glucosinolates separations are more reproducible than with the original polyhydroxyethyl aspartamide columns. Furthermore, the HILIC system that we report herein permits much greater alteration of the mobile phase composition for customized separation of glucosinolates from plant extracts, across a wide spectrum of polarity.

Figures

Figure 1
Figure 1
A. General structure of glucosinolates (GS; β-thioglucoside N-hydroxysulfates); Side chains, R, can be aliphatic, sulfur-containing (thioalkyl), alcoholic, aromatic, indolyl, or additionally glycosylated. Standards used in this paper are show in (B), (C), and (D).
Figure 2
Figure 2
Shift in glucosinolate retention time with acetonitrile content. Equimolar quantities of glucoraphanin (GR) and glucoiberin (GI) were injected on a ZIC-HILIC column as described in the text.

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