Brain-derived neurotrophic factor regulates AMPA receptor trafficking to post-synaptic densities via IP3R and TRPC calcium signaling

FEBS Lett. 2007 May 15;581(10):2047-54. doi: 10.1016/j.febslet.2007.04.041. Epub 2007 Apr 25.


The change in the number of post-synaptic alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA)-type glutamatergic receptors (AMPARs) by neuronal activity is recognized as a molecular basis of synaptic plasticity. Here, we show that Ca(2+) transients evoked by brain-derived neurotrophic factor (BDNF) induce translocation of a subunit of AMPAR, GluR1, but not NMDAR, to the post-synaptic membrane in cultured cortical pyramidal neurons. Among BDNF-induced Ca(2+) transients, that dependent on IP3R was fully required, while store-operated calcium influx through the non-selective cation channel TRPC (transient receptor potential canonical) was partially required for the GluR1 up-regulation, suggesting that spatial and temporal calcium signaling regulate translocation of GluR1 to the polarized membrane domain.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain-Derived Neurotrophic Factor / pharmacology*
  • Calcium Channels / metabolism*
  • Calcium Signaling / drug effects*
  • Dendritic Spines / drug effects
  • Disks Large Homolog 4 Protein
  • Guanylate Kinases
  • Humans
  • Inositol 1,4,5-Trisphosphate Receptors
  • Intracellular Signaling Peptides and Proteins / metabolism
  • Membrane Glycoproteins / metabolism*
  • Membrane Proteins / metabolism
  • Mice
  • Protein Transport / drug effects
  • Pyramidal Cells / cytology
  • Pyramidal Cells / drug effects
  • Receptors, AMPA / metabolism*
  • Receptors, Cytoplasmic and Nuclear / metabolism*
  • Synaptic Membranes / drug effects*
  • TRPC Cation Channels / metabolism*
  • Time Factors


  • Brain-Derived Neurotrophic Factor
  • Calcium Channels
  • Disks Large Homolog 4 Protein
  • Dlg4 protein, mouse
  • Inositol 1,4,5-Trisphosphate Receptors
  • Intracellular Signaling Peptides and Proteins
  • Itpr1 protein, mouse
  • Membrane Glycoproteins
  • Membrane Proteins
  • Receptors, AMPA
  • Receptors, Cytoplasmic and Nuclear
  • TRPC Cation Channels
  • Guanylate Kinases
  • glutamate receptor ionotropic, AMPA 1