Genetic characterization of avian metapneumovirus subtype C isolated from pheasants in a live bird market

Virus Res. 2007 Sep;128(1-2):18-25. doi: 10.1016/j.virusres.2007.03.029. Epub 2007 May 7.


Complete nucleotide sequences of two avian metapneumoviruses (aMPV), designated PL-1 and PL-2, were isolated from pheasants, revealing novel sequences of the first aMPV to be fully sequenced in Korea. The complete genome of both PL-1 and PL-2 was composed of 13,170 nucleotides. Phylogenetic analysis revealed that PL-1 belonged to aMPV subtype C, sharing higher homology in deduced amino acid sequence identities with hMPV, rather than with aMPV subtypes A and B. Replication of PL-1 in experimentally re-infected pheasants was confirmed by reverse transcription (RT)-polymerase chain reaction (PCR). Chickens and mice were experimentally inoculated with PL-1 to test the replication potential of PL-1 in other species. Although one specimen from the nasal turbinates of an inoculated chicken showed a slight trace of viral replication at 3 days post-infection (dpi), all of the infected mice were negative for aMPV by RT-PCR throughout the experiment, suggesting that PL-1 does not readily infect mammals. This is the first report of the isolation and complete genomic sequence of aMPV subtype C originating from pheasants.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chickens / virology
  • Chlorocebus aethiops
  • Galliformes / virology*
  • Korea / epidemiology
  • Metapneumovirus / classification*
  • Metapneumovirus / genetics*
  • Metapneumovirus / isolation & purification
  • Mice
  • Molecular Sequence Data
  • Paramyxoviridae Infections / epidemiology
  • Paramyxoviridae Infections / veterinary*
  • Paramyxoviridae Infections / virology
  • Phylogeny
  • Poultry Diseases / epidemiology
  • Poultry Diseases / virology*
  • Sequence Analysis, DNA
  • Vero Cells

Associated data

  • GENBANK/EF199771
  • GENBANK/EF199772