Zebrafish spata2 is expressed at early developmental stages

Int J Dev Biol. 2007;51(3):241-6. doi: 10.1387/ijdb.062220em.


Spata2 (spermatogenesis-associated protein 2) was originally described as a novel gene involved in the spermatogenic process. In this study, we cloned a potential zebrafish spata2 orthologue. The consensus open reading frame (1650 bp) encodes a polypeptide of 550 amino acids which shares 37% identity with the human SPATA2. Bioinformatic analysis reveals a small pattern PW [KR] KE [YF][RK] which seems to be of particular interest in the light of its strong conservation between SPATA2 and the recently discovered TAMO protein of D. melanogaster. RT-PCR analysis in adult zebrafish tissues revealed that spata2 mRNA has a broad distribution. Whole-mount in situ hybridization demonstrated that spata2 transcripts are maternally derived and becomes strongly localized in the central nervous system at early developmental stages. From 5 dpf, spata2 expression becomes detectable in the gut and pronephric duct epithelium, suggesting a wide tissue function during vertebrate development.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3' Untranslated Regions
  • 5' Untranslated Regions
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • Codon, Terminator
  • Computational Biology
  • Consensus Sequence
  • DNA, Complementary
  • Embryo, Nonmammalian
  • Gene Expression Regulation, Developmental*
  • In Situ Hybridization
  • Male
  • Molecular Sequence Data
  • Open Reading Frames
  • Organ Specificity
  • Polymerase Chain Reaction
  • Protein Structure, Secondary
  • Protein Structure, Tertiary
  • Proteins
  • RNA, Messenger
  • Sequence Homology, Amino Acid
  • Spermatogenesis / genetics
  • Testis / metabolism
  • Tissue Distribution
  • Zebrafish / embryology*
  • Zebrafish Proteins / chemistry
  • Zebrafish Proteins / genetics
  • Zebrafish Proteins / metabolism*


  • 3' Untranslated Regions
  • 5' Untranslated Regions
  • Codon, Terminator
  • DNA, Complementary
  • Proteins
  • RNA, Messenger
  • SPATA2 protein, human
  • Spata2 protein, zebrafish
  • Zebrafish Proteins