Inhibiting HIV-1 integrase by shifting its oligomerization equilibrium

Proc Natl Acad Sci U S A. 2007 May 15;104(20):8316-21. doi: 10.1073/pnas.0700781104. Epub 2007 May 8.


Proteins are involved in various equilibria that play a major role in their activity or regulation. The design of molecules that shift such equilibria is of great therapeutic potential. This fact was demonstrated in the cases of allosteric inhibitors, which shift the equilibrium between active and inactive (R and T) states, and chemical chaperones, which shift folding equilibrium of proteins. Here, we expand these concepts and propose the shifting of oligomerization equilibrium of proteins as a general methodology for drug design. We present a strategy for inhibiting proteins by "shiftides": ligands that specifically bind to an inactive oligomeric state of a disease-related protein and modulate its activity by shifting the oligomerization equilibrium of the protein toward it. We demonstrate the feasibility of our approach for the inhibition of the HIV-1 integrase (IN) protein by using peptides derived from its cellular-binding protein, LEDGF/p75, which specifically inhibit IN activity by a noncompetitive mechanism. The peptides inhibit the DNA-binding of IN by shifting the IN oligomerization equilibrium from the active dimer toward the inactive tetramer, which is unable to catalyze the first integration step of 3' end processing. The LEDGF/p75-derived peptides inhibit the enzymatic activity of IN in vitro and consequently block HIV-1 replication in cells because of the lack of integration. These peptides are promising anti-HIV lead compounds that modulate oligomerization of IN via a previously uncharacterized mechanism, which bears advantages over the conventional interface dimerization inhibitors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Catalysis / drug effects
  • Cells, Cultured
  • Chromatography, Gel
  • DNA, Viral / metabolism
  • Dimerization
  • Fluorescence Polarization
  • HIV Integrase / chemistry*
  • HIV Integrase / metabolism*
  • HIV Integrase Inhibitors / pharmacology*
  • HIV-1 / enzymology
  • HIV-1 / physiology
  • HeLa Cells
  • Humans
  • Intercellular Signaling Peptides and Proteins / chemistry
  • Intercellular Signaling Peptides and Proteins / pharmacology
  • Ligands
  • Models, Biological
  • Molecular Sequence Data
  • Peptides / chemistry
  • Peptides / pharmacology
  • Protein Binding / drug effects
  • Protein Structure, Quaternary
  • Terminal Repeat Sequences
  • Ultracentrifugation
  • Virus Integration / drug effects
  • Virus Replication / drug effects


  • DNA, Viral
  • HIV Integrase Inhibitors
  • Intercellular Signaling Peptides and Proteins
  • Ligands
  • Peptides
  • lens epithelium-derived growth factor
  • HIV Integrase