Forty mouse strain survey of water and sodium intake

Physiol Behav. 2007 Aug 15;91(5):620-31. doi: 10.1016/j.physbeh.2007.03.025. Epub 2007 Apr 1.


We measured voluntary water and sodium intakes of 40 inbred strains of mice. Groups of approximately 10 males and approximately 10 females from each strain received a series of 48-h tests with a choice between a bottle of water and a bottle of one of the following: water, 25, 75, and 225 mM NaCl, 25, 75, and 225 sodium lactate. Sodium solution intakes were influenced by strain, sex, anion and concentration: Nine strains drank significantly more chloride than lactate, and only one strain (I/LnJ) drank significantly more lactate than chloride. The other 30 strains drank similar volumes of chloride and lactate. Sodium intakes were higher in females than males of 8 strains and did not differ by sex in the other 32 strains. Some strains had consistently high sodium intakes and preferred all sodium solutions to water (129S1/SvImJ, MA/MyJ, NZW/LacJ and SWR/J), some showed indifference (i.e. preferences not significantly different from 50%) to all concentrations tested (A/J, C57BL/6J, FVB/NJ and SEA/GnJ), and some had consistently low sodium intakes (AKR/J, C3H/HeJ, C57BL/10J, CBA/J, DBA/2J, I/LnJ, JF1/Ms, LP/J, NON/LtJ, PERA/EiJ, PL/J, and RIIIS/J). The results illustrate the diversity of voluntary sodium intake in mice and will assist in the selection of appropriate strains for focused genetic and physiological analyses.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Oral
  • Analysis of Variance
  • Animal Feed
  • Animals
  • Dose-Response Relationship, Drug
  • Drinking Behavior / physiology*
  • Feeding Behavior / physiology*
  • Female
  • Food Preferences / physiology*
  • Male
  • Mice
  • Mice, Inbred Strains / physiology*
  • Reference Standards
  • Sex Factors
  • Sodium Chloride / administration & dosage
  • Sodium Lactate / administration & dosage
  • Sodium, Dietary / administration & dosage*
  • Species Specificity
  • Water-Electrolyte Balance


  • Sodium, Dietary
  • Sodium Chloride
  • Sodium Lactate