[18S-25S rDNA variation in tissue culture of some Gentiana L. species]

V M Mel'nyk; I O Andrieiev; K V Spiridonova; N M Strashniuk; V A Kunakh

[18S-25S rDNA variation in tissue culture of some Gentiana L. species]

Tsitol Genet. 2007 Mar-Apr;41(2):19-23.

[Article in Ukrainian]

Authors

V M Mel'nyk, I O Andrieiev, K V Spiridonova, N M Strashniuk, V A Kunakh

PMID: 17494339

Abstract

18S-25S rDNA of intact plants and tissue cultures of G. acaulis, G. punctata and G. lutea have been investigated by using blot-hybridization. The decrease of rDNA amount was found in the callus cultures as compared with the plants. In contrast to other species, G. lutea showed intragenome heterogeneity of rRNA genes as well as qualitative rDNA changes in tissue culture, in particular appearance of altered repeats. The relationship between the peculiarities of rRNA gene structure and their rearrangements in in vitro culture was suggested.

Publication types

English Abstract
Research Support, Non-U.S. Gov't

MeSH terms

DNA, Plant / genetics*
DNA, Ribosomal / genetics*
Electrophoresis, Agar Gel
Gentiana / genetics*
In Situ Hybridization
Polymorphism, Restriction Fragment Length*
RNA, Ribosomal / genetics*
RNA, Ribosomal, 18S / genetics*
Tissue Culture Techniques

Substances

DNA, Plant
DNA, Ribosomal
RNA, Ribosomal
RNA, Ribosomal, 18S
RNA, ribosomal, 25S