Microgram-scale protein structure determination by NMR

Nat Methods. 2007 Jun;4(6):491-3. doi: 10.1038/nmeth1051. Epub 2007 May 13.


Using conventional triple-resonance nuclear magnetic resonance (NMR) experiments with a 1 mm triple-resonance microcoil NMR probe, we determined near complete resonance assignments and three-dimensional (3D) structure of the 68-residue Methanosarcina mazei TRAM protein using only 72 mug (6 microl, 1.4 mM) of protein. This first example of a complete solution NMR structure determined using microgram quantities of protein demonstrates the utility of microcoil-probe NMR technologies for protein samples that can be produced in only limited quantities.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Archaeal Proteins / chemistry*
  • Magnetic Resonance Spectroscopy / methods*
  • Methanosarcina / chemistry*
  • Models, Molecular
  • Protein Conformation


  • Archaeal Proteins