Identification and characterization of DUSP27, a novel dual-specific protein phosphatase

FEBS Lett. 2007 May 29;581(13):2527-33. doi: 10.1016/j.febslet.2007.04.059. Epub 2007 Apr 30.

Abstract

A novel human dual-specific protein phosphatase (DSP), designated DUSP27, is here described. The DUSP27 gene contains three exons, rather than the predicted 4-14 exons, and encodes a 220 amino acid protein. DUSP27 is structurally similar to other small DSPs, like VHR and DUSP13. The location of DUSP27 on chromosome 10q22, 50kb upstream of DUSP13, suggests that these two genes arose by gene duplication. DUSP27 is an active enzyme, and its kinetic parameters and were determined. DUSP27 is a cytosolic enzyme, expressed in skeletal muscle, liver and adipose tissue, suggesting its possible role in energy metabolism.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Adipose Tissue / enzymology
  • Base Sequence
  • Conserved Sequence
  • Cytosol / enzymology
  • DNA / chemistry
  • DNA / genetics
  • Dual-Specificity Phosphatases
  • Energy Metabolism
  • Evolution, Molecular
  • Exons
  • Humans
  • Liver / enzymology
  • Molecular Sequence Data
  • Muscle, Skeletal / enzymology
  • Phosphoprotein Phosphatases / genetics*
  • Phosphoprotein Phosphatases / metabolism
  • Plasmids
  • Polymerase Chain Reaction
  • Protein Tyrosine Phosphatases / genetics*
  • Protein Tyrosine Phosphatases / metabolism
  • Recombinant Proteins / metabolism

Substances

  • Recombinant Proteins
  • DNA
  • DUSP13 protein, human
  • Dusp27 protein, human
  • Phosphoprotein Phosphatases
  • Dual-Specificity Phosphatases
  • Protein Tyrosine Phosphatases