A novel mechanism controls the Ca2+ oscillations triggered by activation of ascidian eggs and has an absolute requirement for Cdk1 activity

J Cell Sci. 2007 May 15;120(Pt 10):1763-71. doi: 10.1242/jcs.003012.

Abstract

Fertilisation in ascidians triggers a series of periodic rises in cytosolic Ca(2+) that are essential for release from metaphase I arrest and progression through meiosis II. These sperm-triggered Ca(2+) oscillations are switched off at exit from meiosis II. Ascidian zygotes provided the first demonstration of the positive feedback loop whereby elevated Cdk1 activity maintained these Ca(2+) oscillations. Since then it has been reported that Cdk1 sensitises the type I inositol trisphosphate [Ins(1,4,5)P(3)] receptor in somatic cells, and that sperm-triggered Ca(2+) oscillations in mouse zygotes stop because the forming pronuclei sequester phospholipase C zeta that was delivered to the egg by the fertilising sperm. Here, using enucleation, we demonstrate in ascidian eggs that Ca(2+) spiking stops at the correct time in the absence of pronuclei. Sequestration of sperm factor is therefore not involved in terminating Ca(2+) spiking for these eggs. Instead we found that microinjection of the Cdk1 inhibitor p21 blocked Ca(2+) spiking induced by ascidian sperm extract (ASE). However, such eggs were still capable of releasing Ca(2+) in response to Ins(1,4,5)P(3) receptor agonists, indicating that ASE-triggered Ca(2+) oscillations can stop even though the response to Ins(1,4,5)P(3) remained elevated. These data suggest that Cdk1 activity promotes Ins(1,4,5)P(3) production in the presence of the sperm factor, rather than sensitising the Ca(2+) releasing machinery to Ins(1,4,5)P(3). These findings suggest a new link between this cell cycle kinase and the Ins(1,4,5)P(3) pathway.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biological Clocks / physiology*
  • CDC2 Protein Kinase / metabolism*
  • Calcium Signaling / physiology*
  • Cell Cycle / physiology
  • Cyclin-Dependent Kinase Inhibitor p21 / metabolism
  • Cyclin-Dependent Kinase Inhibitor p21 / pharmacology
  • Female
  • Fertilization / physiology
  • Inositol 1,4,5-Trisphosphate Receptors / drug effects
  • Inositol 1,4,5-Trisphosphate Receptors / metabolism
  • Male
  • Meiosis / physiology*
  • Oocytes / cytology
  • Oocytes / metabolism*
  • Signal Transduction / physiology
  • Sperm-Ovum Interactions / physiology
  • Spermatozoa / metabolism
  • Urochordata / cytology
  • Urochordata / metabolism*

Substances

  • Cyclin-Dependent Kinase Inhibitor p21
  • Inositol 1,4,5-Trisphosphate Receptors
  • CDC2 Protein Kinase