HML-1 antigen on mucosa-associated T cells, activated cells, and hairy leukemic cells is a new integrin containing the beta 7 subunit

Am J Pathol. 1991 Dec;139(6):1297-301.

Abstract

The monoclonal antibodies HML-1, B-ly7 and Ber-ACT8 recognize intramucosal gut T lymphocytes, activated cells, and hairy cell leukemia. The antigen on hairy cells consists of three glycoproteins (160 kappa D, 130 kappa D and 105 kappa D unreduced; 145 kappa D and 120 kappa D reduced). These peptides have biochemical features reminiscent of integrins but we have shown by immunoprecipitation that they are not known integrin subunits. We have used a newly produced antibody (BP6) to purify this molecule and shown by N-terminal sequence analysis that the smallest subunit is the product of integrin beta 7 cDNA. This molecule is thus a new member of the integrin family of leucocyte adhesion proteins. Immunoprecipitation experiments indicate that the two larger subunits are recognized by HML-1, B-ly7 and Ber-ACT8.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Antibodies, Monoclonal
  • Antigens, Neoplasm / analysis*
  • Antigens, Neoplasm / chemistry
  • Biomarkers, Tumor
  • Chromatography, Affinity
  • Humans
  • Integrin beta Chains*
  • Integrins / chemistry
  • Integrins / metabolism*
  • Intestinal Mucosa / metabolism*
  • Leukemia, Hairy Cell / metabolism*
  • Leukemia, Hairy Cell / pathology
  • Lymphocyte Activation*
  • Molecular Sequence Data
  • T-Lymphocytes / metabolism*
  • T-Lymphocytes / physiology

Substances

  • Antibodies, Monoclonal
  • Antigens, Neoplasm
  • Biomarkers, Tumor
  • Integrin beta Chains
  • Integrins
  • integrin alphaEbeta7
  • integrin beta7