Protein oxidation, repair mechanisms and proteolysis in Saccharomyces cerevisiae

IUBMB Life. Apr-May 2007;59(4-5):293-8. doi: 10.1080/15216540701225958.

Abstract

Reactive oxygen species, generated as normal by-products of aerobic metabolism or due to cellular stress, oxidize molecules and cause cell death by apoptosis. The accumulation of oxidized proteins is a hallmark of aging and a number of aging diseases. Oxidation can impair protein function as the proteins are unfolded leading to an increase of protein hydrophobicity and often resulting in the formation of toxic aggregates. The yeast Saccharomyces cerevisiae has been used as a eukaryotic model system to analyze the molecular mechanisms of oxidative stress protection. This paper reviews how the identification in yeast of specific damaged proteins has provided new insights into mechanisms of cytotoxicity and highlights the role of repair and degradative processes, including vacuolar/lysosomal and proteasomal proteolysis, in housekeeping after protein oxidative damage.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Amino Acids / chemistry
  • Lysosomes / metabolism
  • Oxidation-Reduction
  • Oxidative Stress*
  • Proteasome Endopeptidase Complex / metabolism
  • Protein Carbonylation
  • Reactive Oxygen Species / metabolism
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae Proteins / chemistry
  • Saccharomyces cerevisiae Proteins / metabolism*
  • Sulfur / chemistry

Substances

  • Amino Acids
  • Reactive Oxygen Species
  • Saccharomyces cerevisiae Proteins
  • Sulfur
  • Proteasome Endopeptidase Complex