Serum proteomic-based analysis of pancreatic carcinoma for the identification of potential cancer biomarkers

Biochim Biophys Acta. 2007 Jun;1774(6):764-71. doi: 10.1016/j.bbapap.2007.04.001. Epub 2007 Apr 5.


To identify new biomarkers that improve the early diagnosis and lead to possible therapeutic targets in pancreatic carcinoma, we performed a proteomic approach to compare serum protein expression patterns of pancreatic carcinoma patients with that of gastric cancer patients, other pancreatic disease patients, and healthy volunteers. By two-dimensional gel electrophoresis (2-DE) analyses and mass spectroscopic identification, 10 protein spots were found significantly changed in pancreatic carcinoma and 5 proteins including cyclin I, Rab GDP dissociation inhibitor beta (GDI2), alpha-1 antitrypsin precursor, Haptoglobin precursor, and Serotransferrin precursor were successfully identified. The increased levels of cyclin I and GDI2 found to be associated with pancreatic carcinoma were further confirmed by Western blot analyses in an independent series of serum samples and/or pancreatic juice samples. Applying immunohistochemistry, we further validated expression of cyclin I and GDI2 in additional pancreatic carcinomas. These results indicate that cyclin I and GDI2 may be potential molecular targets for pancreatic cancer diagnostics and therapeutics.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomarkers, Tumor / blood*
  • Biomarkers, Tumor / chemistry
  • Blood Proteins / analysis*
  • Blood Proteins / chemistry
  • Blotting, Western
  • Cyclin I
  • Cyclins / blood*
  • Cyclins / chemistry
  • Electrophoresis, Gel, Two-Dimensional
  • Guanine Nucleotide Dissociation Inhibitors / blood*
  • Guanine Nucleotide Dissociation Inhibitors / chemistry
  • Health
  • Humans
  • Immunohistochemistry
  • Middle Aged
  • Pancreatic Neoplasms / blood*
  • Pancreatic Neoplasms / diagnosis*
  • Proteomics
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization


  • Biomarkers, Tumor
  • Blood Proteins
  • CCNI protein, human
  • Cyclin I
  • Cyclins
  • Guanine Nucleotide Dissociation Inhibitors