Development of a novel microarray methodology for the study of SNPs in the promoter region of the TNF-alpha gene: their association with obstructive pulmonary disease in Greek patients

Clin Biochem. 2007 Aug;40(12):843-50. doi: 10.1016/j.clinbiochem.2007.03.024. Epub 2007 Apr 19.


Objectives: Polymorphisms in promoter region of TNF-alpha gene were shown to interfere with the transcriptional activity of the gene resulting in the production of different levels of TNF-alpha product suggesting their involvement in susceptibility or severity of many inflammatory diseases. We set up a case-control study consisting of 117 COPD (Chronic Obstructive Pulmonary Disease), 62 DB (bronchiectasis) patients and two control groups (109 smokers without COPD-healthy smokers control group and 212 general population subjects) to evaluate involvement of TNF-alpha gene polymorphisms in the abovementioned diseases in a homogeneous population.

Methods: The novel methodology of the NanoChip Molecular Biology Workstation (MBW Nanogen was employed to genotype the 5 promoter SNPs.

Results and conclusions: Genotype frequencies of the 5 SNPs showed no significant difference between the COPD and DB patient groups and the healthy smokers group. Statistical difference (p=0.043) was only revealed between the haplotype frequencies in COPD patients compared to the general population control group. The NanoChip MBW is an accurate method for SNP screening.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Algorithms
  • Alleles
  • Case-Control Studies
  • Genetic Predisposition to Disease*
  • Greece
  • Haplotypes
  • Heterozygote
  • Humans
  • Middle Aged
  • Odds Ratio
  • Oligonucleotide Array Sequence Analysis / methods*
  • Polymorphism, Single Nucleotide / genetics*
  • Promoter Regions, Genetic / genetics*
  • Pulmonary Disease, Chronic Obstructive / genetics*
  • Tumor Necrosis Factor-alpha / genetics*
  • White People / genetics*


  • Tumor Necrosis Factor-alpha

Associated data

  • OMIM/191160
  • RefSeq/NM_000594