The new field of membrane rafts has provided fresh insight and a novel framework in which to understand the interaction, relation, and organization of lipids and proteins within cell membranes. This review will examine our current understanding of membrane rafts and their role in human health. In addition, the effect of various lipids, including dietary lipids, on membrane raft structure and function will be discussed. Membrane rafts are found in all cells and are characterized by their high concentration of cholesterol, sphingolipids, and saturated fatty acids. These lipids impart lateral segregation of membrane proteins, thus facilitating the spatial organization and regulation of membrane proteins involved in many cellular processes, such as cell proliferation, apoptosis, and cell signaling. Therefore, membrane rafts are shedding new light on the origins of metabolic disturbances and diseases such as cancer, insulin resistance, inflammation, cardiovascular disease, and Alzheimer's disease, which will be further discussed in this review.