A mouse model of orthotopic vascularized aerated lung transplantation

Am J Transplant. 2007 Jun;7(6):1672-9. doi: 10.1111/j.1600-6143.2007.01819.x.


Outcomes after lung transplantation are markedly inferior to those after other solid organ transplants. A better understanding of cellular and molecular mechanisms contributing to lung graft injury will be critical to improve outcomes. Advances in this field have been hampered by the lack of a mouse model of lung transplantation. Here, we report a mouse model of vascularized aerated single lung transplantation utilizing cuff techniques. We show that syngeneic grafts have normal histological appearance with minimal infiltration of T lymphocytes. Allogeneic grafts show acute cellular rejection with infiltration of T lymphocytes and recipient-type antigen presenting cells. Our data show that we have developed a physiological model of lung transplantation in the mouse, which provides ample opportunity for the study of nonimmune and immune mechanisms that contribute to lung allograft injury.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Lung Transplantation / methods*
  • Lung Transplantation / pathology
  • Mice
  • Mice, Inbred C57BL
  • Models, Animal
  • Pulmonary Artery / pathology
  • Pulmonary Circulation / physiology*
  • Pulmonary Veins / pathology
  • Transplantation, Isogeneic / pathology
  • Transplantation, Isogeneic / physiology