Photochemically induced cerebral ischemia in a mouse model

Surg Neurol. 2007 Jun;67(6):620-5; discussion 625. doi: 10.1016/j.surneu.2006.08.077. Epub 2007 Feb 15.

Abstract

Background: MCAO has been widely used to produce ischemic brain lesions. The lesions induced by MCAO tend to be variable in size because of the variance in the collateral blood supply found in the mouse brain.

Methods: We modified the rat photothrombosis model for use in mice. Male C57BL/6 mice were subjected to focal cerebral ischemia by photothrombosis of cortical microvessels. Cerebral infarction was produced by intraperitoneal injection of rose bengal, a photosensitive dye, and by focal illumination through the skull. Motor impairment was assessed by the accelerating rotarod and staircase tests. The brain was perfusion fixed for histologic determination of infarct volume 4 weeks after stroke.

Results: The lesion was located in the frontal and parietal cortex and the underlying white matter was partly affected. A relatively constant infarct volume was achieved 1 month after photothrombosis. The presence of the photothrombotic lesion significantly impaired the motor performance as measured by the rotarod and staircase tests. Our findings show that photothrombotic infarction in mice is highly reproducible in size and location.

Conclusion: This procedure can provide a simple model of cerebral infarction for a unilateral motor cortex lesion. In addition, it can provide a suitable model for the study of potential neuroprotective and therapeutic agents in human stroke.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain Ischemia* / diagnosis
  • Brain Ischemia* / etiology
  • Brain Ischemia* / physiopathology
  • Disease Models, Animal
  • Fluorescent Dyes / administration & dosage
  • Fluorescent Dyes / adverse effects
  • Frontal Lobe / blood supply
  • Frontal Lobe / physiopathology
  • Injections, Intraperitoneal
  • Intracranial Thrombosis / complications
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Movement Disorders / etiology
  • Parietal Lobe / blood supply
  • Parietal Lobe / physiopathology
  • Photic Stimulation*
  • Rose Bengal / administration & dosage
  • Rose Bengal / adverse effects
  • Severity of Illness Index

Substances

  • Fluorescent Dyes
  • Rose Bengal