Up-regulation of gastric cancer cell invasion by Twist is accompanied by N-cadherin and fibronectin expression

Biochem Biophys Res Commun. 2007 Jul 6;358(3):925-30. doi: 10.1016/j.bbrc.2007.05.023. Epub 2007 May 15.


Twist, a newly found EMT-inducer, has been reported to be up-regulated in those of diffuse-type gastric carcinomas with high N-cadherin level. We show here MKN45, a cell line derived from undifferentiated carcinomas cells, expresses high levels of Twist. Down-regulation of Twist, using an antisense Twist vector in MKN45 cells, inhibits cell migration and invasion, companied with a morphologic changes associated with MET. Suppression of Twist also decreases the expressions of N-cadherin and fibronectin, but not of E-cadherin in MKN45. In contrast, overexpression of Twist in MKN28, a cell line derived from moderate differentiated carcinomas, results in up-regulation of N-cadherin and fibronectin, companied with down-regulation of E-cadherin. Taken together, our results suggest that Twist regulates cell motility and invasion in gastric cancer cell lines, probably through the N-cadherin and fibronectin production.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cadherins / biosynthesis*
  • Carcinoma / metabolism
  • Cell Line, Tumor
  • Cell Movement
  • Fibronectins / biosynthesis*
  • Gene Expression Regulation, Neoplastic*
  • Humans
  • Mice
  • NIH 3T3 Cells
  • Neoplasm Invasiveness
  • Nuclear Proteins / physiology*
  • Stomach Neoplasms / metabolism*
  • Stomach Neoplasms / pathology
  • Transfection
  • Twist-Related Protein 1 / physiology*


  • Cadherins
  • Fibronectins
  • Nuclear Proteins
  • TWIST1 protein, human
  • Twist-Related Protein 1