Abstract
Analysis of human melanocytes and melanoma cell lines for proteins interacting with the octamer control sequence (ATGCAAAT) has revealed two distinct melanoma octamer binding proteins, Oct-M1 and Oct-M2. The latter was restricted to cell lines derived from tumor metastases. The level of Oct-M1 activity in a pigmented melanoma line was enhanced in comparison to the general octamer binding protein Oct-1 when cells were cultured in the presence of the depigmenting agent dithiothreitol and conversely was reduced by the differentiating and pigment inducing agents butyric acid and dimethyl sulfoxide.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Butyrates / pharmacology
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Butyric Acid
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Cell Differentiation / drug effects
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Cell Line, Transformed
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DNA-Binding Proteins / metabolism*
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Dimethyl Sulfoxide / pharmacology
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Dithiothreitol / pharmacology
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Gene Expression Regulation, Neoplastic*
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Host Cell Factor C1
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Humans
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Melanoma / metabolism*
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Octamer Transcription Factor-1
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Transcription Factors / metabolism*
Substances
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Butyrates
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DNA-Binding Proteins
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HCFC1 protein, human
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Host Cell Factor C1
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Octamer Transcription Factor-1
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POU2F1 protein, human
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Transcription Factors
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Butyric Acid
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Dithiothreitol
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Dimethyl Sulfoxide