Androgen influence on cholesterogenic enzyme mRNA levels in the mouse meibomian gland

Curr Eye Res. 2007 May;32(5):393-8. doi: 10.1080/02713680701316674.

Abstract

Purpose: We tested our hypothesis that testosterone increases the meibomian gland gene expression of numerous enzymes in the cholesterol biosynthetic pathway.

Methods: Meibomian glands were obtained from castrated mice treated with vehicle or testosterone for 2 weeks. Tissues were processed for the analysis of selected mRNAs by real-time PCR.

Results: Our research demonstrates that testosterone stimulates a significant increase in the mRNA levels of mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylase, isopentenyl pyrophosphate isomerase, geranylgeranyl pyrophosphate synthase, squalene epoxidase, lanosterol synthase, lanosterol demethylase, and Delta 7-sterol reductase.

Conclusions: Our findings indicate that androgens may promote cholesterol biosynthesis in the meibomian gland.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cholesterol / biosynthesis*
  • Enzymes / genetics*
  • Enzymes / metabolism*
  • Gene Expression / drug effects
  • Male
  • Meibomian Glands / metabolism*
  • Mice
  • Mice, Inbred BALB C
  • RNA, Messenger / metabolism*
  • Testosterone / pharmacology*

Substances

  • Enzymes
  • RNA, Messenger
  • Testosterone
  • Cholesterol