Global gene expression in Ha-ras and B-raf mutated mouse liver tumors

Int J Cancer. 2007 Sep 15;121(6):1382-5. doi: 10.1002/ijc.22801.


Chemically-induced mouse liver tumors harbor mutations in different oncogenes. About 50% of tumors contain activating mutations in the Ha-ras gene contain and about 20% of tumors show point mutations in the B-raf oncogene. We have investigated the gene expression profiles in tumors of the 2 genotypes by microarray analysis. In total, approximately 500 genes or expressed sequences were aberrantly expressed in tumors relative to normal liver tissue. Around two/thirds of them were significantly altered in both Ha-ras and B-raf mutated liver tumors, and most of the remaining genes showed at least qualitatively comparable changes in both tumor types. Several functional clusters were hypothesized in tumors of the 2 genotypes which involve alterations in a battery of genes encoding enzymes of lipid metabolism. The similarity in the patterns of global gene expression of Ha-ras and B-raf mutated liver tumors suggests that mutational activation of the 2 oncogenes results in activation of a common set of transcriptional regulators.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkylating Agents / toxicity
  • Animals
  • Diethylnitrosamine / toxicity
  • Gene Expression
  • Gene Expression Profiling
  • Genes, ras / genetics*
  • Liver Neoplasms, Experimental / chemically induced
  • Liver Neoplasms, Experimental / genetics*
  • Mice
  • Mice, Inbred C3H
  • Mutation
  • Oligonucleotide Array Sequence Analysis
  • Proto-Oncogene Proteins B-raf / genetics*
  • Reverse Transcriptase Polymerase Chain Reaction


  • Alkylating Agents
  • Diethylnitrosamine
  • Braf protein, mouse
  • Proto-Oncogene Proteins B-raf