Structural elucidation of a novel phosphoglycolipid isolated from six species of Halomonas

J Lipid Res. 2007 Aug;48(8):1825-31. doi: 10.1194/jlr.M700152-JLR200. Epub 2007 May 22.

Abstract

The structure of a new phosphoglycolipid from the halophilic Gram-negative bacteria Halomonas elongata ATCC 33173(T), Halomonas eurihalina ATCC 49336(T), Halomonas almeriensis CECT 7050(T), strain Sharm (AM238662), Halomonas halophila DSM 4770(T), and Halomonas salina ATCC 49509(T) was elucidated by NMR and mass spectroscopy studies. In all of the species examined, the polar lipid composition consisted of 1,2-diacylglycero-3-phosphorylethanolamine, 1,2-diacylglycero-3-phosphoryl-glycerol, bisphosphatidyl glycerol, and the new phosphoglycolipid PGL1. The structure of PGL1 was established to be (2-(alpha-D-glucopyranosyloxy)-3-hydroxy-propyl)-phosphatidyl diacylglycerol. C16:0;C18:1 and C16:0;C19:cyclopropane are the most abundant acyl chains linked to the phosphatidylglycerol moiety of each isolated PGL1. All of the species presenting the lipid PGL1 belong to Halomonas rRNA group 1, suggesting that the new phosphoglycolipid could be a chemotaxonomic marker of this phylogenetic group.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Glycolipids / chemistry*
  • Halomonas / chemistry*
  • Halomonas / classification
  • Magnetic Resonance Spectroscopy
  • Mass Spectrometry
  • Molecular Sequence Data
  • Phosphatidylglycerols / chemistry*
  • Phospholipids / chemistry*
  • Phylogeny

Substances

  • (2-glucopyranosyloxy-3-hydroxypropyl)phosphatidyl diacylglycerol
  • Glycolipids
  • Phosphatidylglycerols
  • Phospholipids

Associated data

  • GENBANK/AM238662