There is limited information about chlamydia prevalence in men. Our study aimed to measure the prevalence of chlamydia in 16-29-year-old heterosexual men. Recruitment was undertaken through urban sporting clubs. Participants were approached after training to complete a questionnaire and to provide a urine sample that was tested for Chlamydia trachomatis. Participants with positive results received treatment. Fifty participants were recruited and four were found to be infected (8.5%, 95% CI 2.76-21.27). There was a high prevalence of C. trachomatis in our study participants, highlighting the importance of including men in chlamydia response programs.