Enhanced sensitivity of FRET-based protease sensors by redesign of the GFP dimerization interface

Chembiochem. 2007 Jul 9;8(10):1119-21. doi: 10.1002/cbic.200700109.

Authors

Jan L Vinkenborg¹, Toon H Evers, Sanne W A Reulen, E W Meijer, Maarten Merkx

Affiliation

¹ Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands.

PMID: 17525917
DOI: 10.1002/cbic.200700109

No abstract available

MeSH terms

Bacterial Proteins / metabolism
Dimerization
Endopeptidases / chemistry
Fluorescence Resonance Energy Transfer / instrumentation*
Fluorescence Resonance Energy Transfer / methods
Green Fluorescent Proteins / chemistry
Green Fluorescent Proteins / metabolism
Indicators and Reagents / pharmacology
Luminescent Proteins / metabolism
Mutation
Peptide Hydrolases / chemistry*
Peptides / chemistry
Protein Structure, Tertiary
Sensitivity and Specificity
Time Factors

Substances

Bacterial Proteins
Cyan Fluorescent Protein
Indicators and Reagents
Luminescent Proteins
Peptides
yellow fluorescent protein, Bacteria
Green Fluorescent Proteins
Endopeptidases
Peptide Hydrolases